Coding

Part:BBa_K1484103

Designed by: Guy Lewy   Group: iGEM14_Cambridge-JIC   (2014-10-08)

Benzalacetone Synthase, compatible with MoClo

This enzyme catalyses the condensation of p-coumaroyl coA with malonyl coA to form p-hydroxybenzalacetone. This is an important step in the biosynthesis of raspberry ketone, the main odour and flavour constituent of raspberries.

RK_pathway_sma.jpg

This enzyme was originally cloned from Rheum palmatum (Chinese rhubarb) by Ikuro Abe at The School of Pharmaceutical Sciences, University of Shizuoka, Japan.


If interested in making Raspberry Ketone or Benzalacetone - take a look at our benzalacetone generator (BBa_K1484317)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1080
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1169


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Categories
Parameters
None